Regulatory

Part:BBa_J100550:Design

Designed by: Cathleen Krabak   Group: Campbell M Lab   (2020-03-26)


Variation in Constitutively Functioning AHL Repressible Promoter in E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 35
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We decided to change one new nucleotide from the original sequence used in the paper above to see if RFP production would be altered.


Source

This promoter came from an article published under this link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4444344/

References